Deprotection of Tert-Butyloxycarbonyl (N-BOC) Groups Using a Microwave
Do you have a protected amine research chemical that can't be ingested on its own due to amine protection? If yes, then this is the guide for you! A fair warning that this method produces non-toxic byproducts such as sodium carbonate and/or unreacted hydrocarbons which may be toxic. There are ways to get rid of these through purification through acetone. This method may be in the next guide.
Now onto the good stuff:
Firstly, obtain either 95% everclear or anhydrous isopropyl alcohol (preferred due to ease of drying). Dissolve 0.334 millimolar equivalent of your protected amine into 2ml of your solvent of choice in a 10ml borosilicate beaker. If you can't get it to dissolve you can heat it mildly though be warned you may need to add more solvent to get back to 2ml due to evaporation. Add 29mg (0.334mmol/equimolar quantities) of baking soda to the solution and allow it to dissolve (arm and hammer is best because it contains no additives and is only pure sodium bicarbonate).
Next add your beaker into the microwave reactor (any microwave works for this though you'll have to do some calculations based on your microwaves power in wattage). Place some sort of covering over top of the beaker (saran wrap is good). Set that bitch to 300 watts and cook for 5 minutes making sure to observe the process in the rare case of fires. After, allow the alcohol to evaporate in an open dish.
That's it! You have a safe product to vaporize and the expected yield is an astonishing 95%!
Notes on the microwave process:
You need a total of 300 watts output power at 2450 MHz which is standard frequency for microwaves- though anything around this works well. The power settings on microwaves are percentages of the expected output power. Level three being 30% as seen in the guide which is 1000*0.3=300. If your microwave is a different wattage- do whatever percentage is closest to 300 watts.
Notes on the molar equivalencies:
I'm guessing N-BOC-DMT's formula is C17H24N2O2 or SMILES CN(CCC1=CNC2=C1C=CC=C2)CC(=O)OC(C)(C)C and thusly has a molar mass of 288.38466 g/mol, though I'm unsure of this because I couldn't find it online. Either way the molar mass of your compound can be put into this calculator
https://calculator-online.net/moles-to-grams-calculator/ all you have to do is add the g/mol mass and the number 0.334 in the moles area when calculating mass in grams. Make sure to put the moles as mmol and not mol!
Citation:
https://www.sciencedirect.com/science/article/abs/pii/S0040403908023502
Enjoy!
-Trimipramine
